In Vitro Radiation Response of Cells from Four Human Tumors Propagated in Immune-suppressed Mice1

In vitro radiation response of cells from four human tumors propagated in immune-suppressed mice.

Two recently developed clonogenic assays for human tumor cells have been used to measure the in vitro radiation cell survival of four human tumors, a pancreatic carcinoma, a colonic carcinoma, an oat cell carcinoma of the lung, and a melanoma, propagated as xenografts in immune-suppressed mice. The slopes and shoulders of the survival curves for the first three tumors were all similar with Do's...

Immune response of chickens to four lentogenic strains of Newcastle disease virus propagated in lamb kidney cell cultures

Immune Elimination of Host Fibroblasts for the Cultivation of Human Tumors Transplanted into Nude Mice1

We report here a useful method for the isolation and culti vation of human tumor cells in vitro from human tumors grown in nude mice. A rabbit was immunized with spleen cells ob tained from adult nude mice. The rabbit antiserum in the presence of complement effectively killed cultured cells derived from various mouse tissues, but it was not cytotoxic to cultured cells from human tissues includi...

Quantitation of genome damage and transcriptional profile of DNA damage response genes in human peripheral blood mononuclear cells exposed in vitro to low doses of neutron radiation

Background: Humans are exposed to ionizing radiation from different sources that include natural, occupational, medical, accidental exposures. Evaluation of the effect of low level of neutron exposure to human cells in vitro has important implications to human health. Attempts were made to measure genome damage, transcriptional profile of DNA damage response and repair genes in peripheral blood...

Xenografts of Human Bladder Cancer in Immune-Deprived Mice1

Human bladder cancer from cystoscopic biopsies and from established cell lines was transplanted into mice that were immune suppressed by thymectomy plus sequential treatment with 1-ß-0-arabinofuranosylcytosine and whole-body irradia tion. Each of four established human bladder cancer cell lines generated transplantable tumors in these mice, and some of the mice developed pulmonary métastases....